NEIL1 Antibody
NEIL1 Antibody
Product Specifications
Product Name Alternative
NEIL1, NEH1, nei endonuclease VIII-like 1 (E. coli), NEI1, FLJ22402, endonuclease VIII, FPG1, hFPG1, nei endonuclease VIII-like 1
UniProt
Q96FI4
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-DIPSLEPEGTSAS
Target
NEIL1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1μg/mL. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10μg/mL.
Prediction Reactivity
Bovine, Porcine
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_078884.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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