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NBEA Antibody

NBEA Antibody

Product Specifications

Product Name Alternative

NBEA, neurobeachin, RP11-270C18.1, BCL8B, FLJ10197, KIAA1544, LYST2, lysosomal trafficking regulator, lysosomal trafficking regulator 2

UniProt

Q8NFP9

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-DFNRWHYEHQNRY

Target

NBEA

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 300kDa band observed in lysates of transfected HEK293 and endogenously in lysates of insulinoma cell line B-TC3 (calculated MW of 327kDa according to NP_056493.3) . Data from a previous goat. Data kindly provided by Professor John Creemers, K.U. Leuven, Belgium. Recommended concentration: 1-3μg/mL.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows textured cytoplasm staining in the neuronal cell bodies. Recommended concentration: 5-10μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_001191126.1, NP_056493.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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