NFIL3 Antibody

Product Specifications

Product Name Alternative

NFIL3, nuclear factor, interleukin 3 regulated, E4BP4, IL3BP1, NFIL3A, NF-IL3A, interleukin-3 promoter transcriptional activator, OTTHUMP00000021633

UniProt

Q16649

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-RLIATQPISASDSG

Target

NFIL3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 60kDa band observed in nuclear lysates of cell line Jurkat and HeLa (calculated MW of 51.5kDa according to NP_005375.2) . The observed molecular weight corresponds to different antibodies from other commercial sources. An additional band of 30kDa was observed in lysates of cell line HeLa, which was completely blocked with the immunizing peptide. Recommended concentration: 0.5-2μg/mL. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, FC, WB

NCBI Accession Number

NP_005375.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.