NONO Antibody
NONO Antibody
Product Specifications
Product Name Alternative
NONO, P54NRB , non-POU domain containing, octamer-binding , HGNC:7871, NMT55, NRB54, P54, Nuclear RNA-binding protein, 54-kD, non-POU-domain-containing, octamer-binding, non-Pou domain-containing octamer (ATGCAAAT) binding protein
UniProt
Q15233
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-NRAAPGAEFAPNK
Target
NONO
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 60kDa band observed in Mouse Brain lysates (calculated MW of 54.2kDa according to Human NP_031389.3 and 54.5kDa according to Mouse NP_075633.2) . Recommended concentration: 0.1-0.3μg/mL.Immunohistochemistry:Paraffin embedded Human Spleen and Testis. Recommended concentration: 3.75μg/mL.
Prediction Reactivity
Canine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_031389.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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