NOTCH3 Antibody

Product Specifications

Product Name Alternative

NOTCH3, Notch homolog 3 (Drosophila), HGNC:7883, CADASIL, CASIL , Notch (Drosophila) homolog 3, Notch homolog 3

UniProt

Q9UM47

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-QLGPQPEVTPKRQ

Target

NOTCH3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave approx 60+70kDa bands in human brain lysates after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 244kDa according to NP_000426.2. The 60+70kDa bands were successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Hippocampus shows select nuclear staining and weaker staining in different comparments of the neuronal cell bodies. Recommended concentration: 4-6μg/mL. Paraffin embedded Human Placenta. Recommended concentration: 5μg/mL.

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_000426.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.