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NRAS Antibody

NRAS Antibody

Product Specifications

Product Name Alternative

NRAS , neuroblastoma RAS viral (v-ras) oncogene homolog , N-ras , NRAS1, N-ras protein part 4 , v-ras neuroblastoma RAS viral oncogene homolog

UniProt

P01111

Reactivity

Human, Rat

Immunogen

The immunogen for this antibody is: C-RMKKLNSSDDGTQ

Target

NRAS

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 21kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2kDa according to NP_002515.1) . Recommended concentration: 0.01-0.03μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3 for this particular batch.Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration: 2-3μg/mL.

Prediction Reactivity

Canine, Mouse

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_002515.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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