What is the catalog number for P4HA1 Antibody?

The catalog number for P4HA1 Antibody is orb1247152.

What sizes are available for P4HA1 Antibody?

P4HA1 Antibody is available in the following size(s): 0.1 mg.

Is P4HA1 Antibody in stock?

P4HA1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

P4HA1 Antibody

Product Specifications

Product Name Alternative

P4HA1, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I, 4-PH alpha-1, P4HA , prolyl 4-hydroxylase, alpha I subunit

UniProt

P13674

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-DFARKDEPDAFKE

Target

P4HA1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in lysates of cell lines HEK293 and A431 (calculated MW of 61.0kDa according to NP_000908.2 and NP_001017962.1) . Recommended concentration: 0.1-0.1μg/mL. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skin and Spleen. Recommended concentration: 5μg/mL.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_001017962.1, NP_000908.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.