PVALB Antibody

Product Specifications

Product Name Alternative

Parvalbumin, HGNC:9704, PVALB, D22S749, parvalbumin alpha, MGC116759

UniProt

P20472

Reactivity

Human, Rat

Immunogen

The immunogen for this antibody is: C-GVDEFSTLVAES

Target

PVALB

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 14-15kDa band observed in Human (Cerebellum) Brain lysates (calculated MW of 12.1kDa according to NP_002845.1) . Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3μg/mL. Approx 10-12kDa band also observed in Rat Skeletal Muscle and in Rat Heart cells transfected with human PVALB, but not in untransfected Rat Heart cells. Data kindly provided by Dr Wang Wang, University of Michigan.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 2.5μg/mL.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10μg/mL.

Tested Applications

ELISA, FC, IHC, WB

NCBI Accession Number

NP_002845.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.