PAX3 Antibody
PAX3 Antibody
Product Specifications
Product Name Alternative
PAX3, WS1, CDHS, HUP2, paired box gene 3 (Waardenburg syndrome 1), paired domain gene 3, paired box homeotic gene 3 (Waardenburg syndrome 1), paired box 3, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, paired box homeotic gene 3, paired domain gene HuP2
UniProt
P23760
Reactivity
Human
Immunogen
The immunogen for this antibody is: TTLAGAVPRMM-C
Target
PAX3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 45kDa band observed in lysates of Human Duodenum, Skin, and in lysates of cell lines A431 (calculated MW of 45.2kDa according to NP_852125.1 and of 44.8kDa according to NP_852126.1) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Esophagus expression of PAX3 is detected in squamous epithelium. Recommended concentration: 4.5μg/mL.
Prediction Reactivity
Bovine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_852123.1, NP_000429.2, NP_852122.1, NP_039230.1, NP_852125.1, NP_852126.1, NP_852124.1, NP_001120838.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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