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PAX8 Antibody

PAX8 Antibody

Product Specifications

Product Name Alternative

PAX8, paired box gene 8, paired domain gene 8, paired box 8

UniProt

Q06710

Reactivity

Human

Immunogen

The immunogen for this antibody is: PHNSIRSGHGGLN-C

Target

PAX8

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Kidney lysates. An additional 48kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9kDa according to Human NP_039247.1 PAX8D and 48.2kDa according to Human NP_003457.1 PAXA) . Recommended concentration: 1-2μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney Cortex. Recommended concentration: 5μg/mL.

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_039247.1, NP_003457.1, NP_039245.1, NP_039246.1, NP_054698.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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