PAX8 Antibody
PAX8 Antibody
Product Specifications
Product Name Alternative
PAX8, paired box gene 8, paired domain gene 8, paired box 8
UniProt
Q06710
Reactivity
Human
Immunogen
The immunogen for this antibody is: PHNSIRSGHGGLN-C
Target
PAX8
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 35kDa band observed in Human Kidney lysates. An additional 48kDa was also observed in some Kidney and HeLa cell lysates (calculated MW of 34.9kDa according to Human NP_039247.1 PAX8D and 48.2kDa according to Human NP_003457.1 PAXA) . Recommended concentration: 1-2μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:Paraffin embedded Human Kidney Cortex. Recommended concentration: 5μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_039247.1, NP_003457.1, NP_039245.1, NP_039246.1, NP_054698.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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