PDE4D Antibody

Product Specifications

Product Name Alternative

PDE4D, phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila), HGNC:8783, DPDE3, HSPDE4D, PDE4DN2, STRK1, cAMP-specific phosphodiesterase 4D, cAMP-specific phosphodiesterase PDE4D6, dunce-like phosphodiesterase E3, phosphodiesterase 4D, cAMP-specific (dunce (Drosophila) -homolog phosphodiesterase E3), DKFZp686M11213, FLJ97311, phosphodiesterase 4D

UniProt

Q08499

Reactivity

Human

Immunogen

The immunogen for this antibody is: QPEACVIDDRSPDT

Target

PDE4D

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. Calculated MW of 91.1kDa according to PDE4D1 (NP_001098101.1), 76.5kDa according to PDE4D2 (NP_006194.2) and 84.7kDa according to PDE4D3 (NP_001159371.1) . Recommended concentration: 0.1-0.3μg/mL.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration: 5μg/mL.ELISA: This antibody has been successfully used in ELISA as described in the following paper: Bazhin et al, Mol Immunol. 2010 Jan;47 (4) :678-84.; PMID: 19939455.

Tested Applications

EIA, ELISA, IHC, WB

NCBI Accession Number

NP_001184151.1, NP_001184150.1, NP_001098101.1, NP_001159371.1, NP_001184152.1, NP_006194.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.