PIK3CA Antibody
PIK3CA Antibody
Product Specifications
Product Name Alternative
PIK3CA, p110-alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide , MGC142161, MGC142163, PI3K, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
UniProt
P42337
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-HQWLKDKNKGEIYD
Target
PIK3CA
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1μg/mL) .Immunohistochemistry:In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration: 2-3μg/mL. Paraffin embedded Human Heart. Recommended concentration: 5μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_006209.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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