RAD51AP1 Antibody
RAD51AP1 Antibody
Product Specifications
Product Name Alternative
RAD51AP1, PIR51, RAD51-interacting protein, RAD51 associated protein 1
UniProt
Q96B01
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-ARVKPLHPNATST
Target
RAD51AP1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 25kDa band in Human Testis lysates at 0.2μg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 39kDda according to NP_006470. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration, 4-6μg/mL.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_006470, NP_001124334.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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