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RAD51AP1 Antibody

RAD51AP1 Antibody

Product Specifications

Product Name Alternative

RAD51AP1, PIR51, RAD51-interacting protein, RAD51 associated protein 1

UniProt

Q96B01

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-ARVKPLHPNATST

Target

RAD51AP1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 25kDa band in Human Testis lysates at 0.2μg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 39kDda according to NP_006470. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining in spermatocytes and spermatids. Recommended concentration, 4-6μg/mL.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_006470, NP_001124334.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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