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PIR Antibody

PIR Antibody

Product Specifications

Product Name Alternative

PIR, pirin, pirin (iron-binding nuclear protein)

UniProt

O00625

Reactivity

Human

Immunogen

The immunogen for this antibody is: GSSKKVTLSVLSREC

Target

PIR

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1) . Recommended concentration: 0.3 - 1.0 μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Spleen. Recommended concentration: 5μg/mL.

Prediction Reactivity

Mouse

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_003653.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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