PIR Antibody
PIR Antibody
Product Specifications
Product Name Alternative
PIR, pirin, pirin (iron-binding nuclear protein)
UniProt
O00625
Reactivity
Human
Immunogen
The immunogen for this antibody is: GSSKKVTLSVLSREC
Target
PIR
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1) . Recommended concentration: 0.3 - 1.0 μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Spleen. Recommended concentration: 5μg/mL.
Prediction Reactivity
Mouse
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_003653.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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