PODXL Antibody

Product Specifications

Product Name Alternative

PODXL, podocalyxin-like, Gp200, MGC138240, PCLP

UniProt

O00592

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-DNLTKDDLDEEEDTH

Target

PODXL

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 160kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 58.6kDa according to NP_001018121.1 and 55.4kDa according to NP_005388.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kershaw et al., J Biol Chem. 1997 Jun 20;272 (25) :15708-14.; PMID: 9188463) . Recommended concentration: 1-3μg/mL.

Prediction Reactivity

Canine

Tested Applications

ELISA, WB

NCBI Accession Number

NP_001018121.1, NP_005388.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.