Anti-p62dok Antibody

Rabbit Polyclonal Antibody specific to DOK (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Downstream of tyrosine kinase 1, p62(dok, pp62

Gene Name

DOK1

Gene ID

1796

NCBI Gene ID

UniProt

Q99704

Accession Number

NP_001184189

Cellular Locus

[Isoform 1]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm, perinuclear region.

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 425- 439 of human p62dok.

Target Antigen

Docking protein 1

Target

p62dok

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3. {PubMed:18156175}.

Additionnal Information

Immunoblotting: use at 1:1,000-1:2,000 dilution. <br><br>Positive control: Whole cell lysate of Jurkat cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human p62dok (62kD).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates which mediates the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF, and insulin receptors, has been identified in human and mouse. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok, along with p56dok, represents a new family of RasGAP-binding proteins.