PTBP1 Antibody
PTBP1 Antibody
Product Specifications
Product Name Alternative
PTBP1, PTB, polypyrimidine tract binding protein 1, PTB2, PTB3, PTB4, pPTB, HNRPI, PTB-1, HNRNPI, RNA binding protein, heterogeneous nuclear ribonucleoprotein polypeptide I, polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I), HNRNP-I, MGC10830, MGC8461, RNA-binding protein, heterogeneous nuclear ribonucleoprotein I
UniProt
P17225
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: DGIVPDIAVGTKR-C
Target
PTBP1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in Human, Mouse and Rat Ovary lysates and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat, HEK293 and 3T3N (calculated MW of 57.2kDa according to NP_114368.1) . Recommended concentration: 0.01-0.3μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Liver and Ovary for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration: 2-4μg/mL.
Prediction Reactivity
Bovine, Canine, Porcine
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_114367.1, NP_787041.1, NP_114368.1, NP_002810.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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