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PTCH1 Antibody

PTCH1 Antibody

Product Specifications

Product Name Alternative

PTCH1, PTCH, patched homolog (Drosophila), HGNC:9585, BCNS, HPE7, NBCCS, PTC, PTC1, patched, patched (Drosophila) homolog, patched homolog 1 (Drosophila), RP11-435O5.3, FLJ26746, FLJ42602, PTCH11, OTTHUMP00000021709, OTTHUMP00000021710, PTCH protein +12b, PTCH protein +4', PTCH protein -10

UniProt

Q13635

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: ELQDVECEERPR

Target

PTCH1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Immunohistochemistry:In paraffin embedded Human Colon shows textured nuclear staining of the absorptive cells. Recommended concentration: 3-6μg/mL. Paraffin embedded Human Breast. Recommended concentration: 3.75μg/mL. This produt has been successfully used in IHC on Human: Abiria SA et al, J Neurooncol. 2014 May 28. PMID: 24867209.Immunofluorescence: Expression of the protein seen in the cytoplasm of HeLa and in the Golgi/membranes of NIH3T3 cells. Recommended concentration: 5μg/mL. This produt has been successfully used in IF on Human: Abiria SA et al, J Neurooncol. 2014 May 28. PMID: 24867209.

Prediction Reactivity

Rat

Tested Applications

ELISA, IF, IHC

NCBI Accession Number

NP_001077072.1, NP_001077073.1, NP_001077071.1, NP_001077076.1, NP_001077075.1, NP_000255.1, NP_001077074.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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