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PTCH Antibody

PTCH Antibody

Product Specifications

Product Name Alternative

PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1 , PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog

UniProt

Q13635

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-HPESRHHPPSNPRQQ

Target

PTCH

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 153.9kDa according to NP_001077071.1) . Recommended concentration: 1-3μg/mL. Primary incubation 1 hour at room temperature. Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5μg/mL.

Prediction Reactivity

Bovine, Canine, Porcine

Tested Applications

ELISA, IF, WB

NCBI Accession Number

NP_001077071.1, NP_001077072.1, NP_001077075.1, NP_000255.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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