ATP6AP2 Antibody
ATP6AP2 Antibody
Product Specifications
Product Name Alternative
ATP6AP2, ATP6IP2, ATPase, H+ transporting, lysosomal interacting protein 2, M8-9, APT6M8-9, ATP6M8-9, renin receptor, V-ATPase M8.9 subunit, ATPase membrane sector associated protein M8-9, vacuolar ATP synthase membrane sector associated protein M8-9, ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9, ELDF10, HT028, MGC99577, MRXE, MSTP009, XMRE, ATPase, H+ transporting, lysosomal interacting protein 2, embryonic liver differentiation factor 10
UniProt
O75787
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-SIIYRMTNQKIRMD
Target
ATP6AP2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Mouse Kidney lysates (calculated MW of 39.1kDa according to NP_081715.1) . An additional band of 45kDa was also observed in some lysates, whch was completely blocked by the immunizing peptide. Recommended concentration:1-2μg/mL. Primary incubation 1 hour at room temperature.Immunohistochemistry:This antibody has been successfully used on Human tissue in the following papers: Pringle et al, J Renin Angiotensin Aldosterone Syst. 2010 Aug 11, PMID: 20702505. Delforce et al, Endocr Connect. 2017 Jan;6 (1) :9-19, PMID: 27956412.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Rat
Tested Applications
ELISA, FC, IHC, WB
NCBI Accession Number
NP_005756.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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