RHPN2 Antibody

RHPN2, p76RBE, rhophilin, Rho GTPase binding protein 2, rhophilin 2, rhophilin-2, RhoB effector, rhophilin-like protein, P76RBE, RHOBP, 76 kDa RhoB effector protein, GTP-Rho-binding protein 2, rhophilin-like Rho-GTPase binding protein

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 35kDa band in Human Pancreas and Prostate lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77kDa according to NP_149094.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows staining of cellular membrane and cytoplasm in convoluted tubular cells. Recommended concentration 5-10μg/mL.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.