RNF213 Antibody
RNF213 Antibody
Product Specifications
Product Name Alternative
RNF213, C17orf27, ring finger protein 213, chromosome 17 open reading frame 27, KIAA1554
UniProt
Q63HN8
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-KTAAVLKWNRE
Target
RNF213
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded human kidney shows rare staining of nucleoli across the kidney . Recommended concentration, 2.5μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_065965.4
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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