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RNF8 Antibody

RNF8 Antibody

Product Specifications

Product Name Alternative

RNF8, ring finger protein 8, ring finger protein (C3HC4 type) 8, KIAA0646, FLJ12013, C3HC4-type zinc finger protein, OTTHUMP00000039684, UBC13/UEV-interacting ring finger protein

UniProt

O76064

Reactivity

Human

Immunogen

The immunogen for this antibody is: GEPGFFVTGDRAG-C

Target

RNF8

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Testis and Lung lysates and approx. 50kDa in nuclear lysates of cell lines HepG2 and Jurkat (predicted MW of 56kDa according to NP_003949.1 and 50.8kDa according to NP_898901.1) . Recommended concentration 0.5-2μg/mL. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.

Tested Applications

ELISA, FC, WB

NCBI Accession Number

NP_898901.1, NP_003949.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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