TRIM21 Antibody

TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro), tripartite motif protein TRIM21, tripartite motif protein 21

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:In transfected HEK293 transiently expressing full-length Human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.2-1μg/mL. Primary incubations were for 1 hour.Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL. Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.