Robo1 Antibody

Product Specifications

Product Name Alternative

Robo1, roundabout homolog 1 (Drosophila), AW494633, AW742721, DUTT1 , roundabout homolog 1

UniProt

O89026

Reactivity

Mouse

Immunogen

The immunogen for this antibody is: C-QKARPAKKQKHQ

Target

Robo1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 200kDa band observed in fetal Mouse Brain lysates (calculated MW of 176kDa according to NP_062286.2) . Recommended concentration: 0.05-0.2μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Mouse Testis. Recommended concentration: 5μg/mL.

Prediction Reactivity

Canine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_062286.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.