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RPE65 Antibody

RPE65 Antibody

Product Specifications

Product Name Alternative

MRPE65, sRPE65, retinal pigment epithelium-specific protein 65kDa, LCA2, RP20, rd12, RBP-binding membrane protein, p63, retinal pigment epithelium-specific 61 kDa protein, retinal pigment epithelium-specific protein (65kD), retinitis pigmentosa 20

UniProt

Q16518

Reactivity

Rat

Immunogen

The immunogen for this antibody is: C-EVKKNARKAPQPE

Target

RPE65

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Rat Retina lysates (calculated MW of 60.9kDa according to Human NP_000320.1 and 61.0kDa according to Rat NP_446014.1) . Recommended concentration: 0.5-1.5μg/mL. Primary incubation was 1 hour.

Prediction Reactivity

Canine, Human

Tested Applications

ELISA, WB

NCBI Accession Number

NP_000320.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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