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UBASH3A Antibody

UBASH3A Antibody

Product Specifications

Product Name Alternative

TULA , STS2 CBL-interacting protein 4, UBASH3A, ubiquitin associated and SH3 domain containing, A , T-cell ubiquitin ligand protein, Suppressor of T-cell receptor signaling 2, CLIP4

UniProt

P57075

Reactivity

Human

Immunogen

The immunogen for this antibody is: QQCSTPDGKYYRPD

Target

UBASH3A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 48kDa band in lysates of B-cell line Daudi after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.1kDa according to NP_061834.1 and 69.8kDa according to NP_001001895.1. The 48kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Spleen shows cytoplasm staining of T-cells eccentrically gathered around an artery. Recommended concentration: 2.5μg/mL.

Prediction Reactivity

Canine

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_061834.1, NP_001001895.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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