MAP3K7 Antibody

MAP3K7 , TAK1, mitogen-activated protein kinase kinase kinase 7 , TGF1a , TGF-beta activated kinase 1, transforming growth factor-beta-activated kinase 1

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa, Daudi and NIH3T3, and approx. 80kDa in lysates of cell line U937 (calculated MW of 67.2 according to Human NP_663304.1 and Mouse NP_033342.1) . The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 1-3μg/mL. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL.

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.