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Tcfec Antibody

Tcfec Antibody

Product Specifications

Product Name Alternative

Tcfec, transcription factor EC , BB107417, MGC107474, TFEC

UniProt

Q9WTW4

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-HITKQQTHPERN

Target

Tcfec

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave one band at approx 37kDa and much stronger bands at approx 70+28kDa in Mouse Testis lysates after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the stonger bands we observe given the calculated size of 35.1kDa according to Mouse NP_112475.1. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) .Immunohistochemistry:In paraffin embedded Mouse Thymus shows nuclear staining in sporadic cells of the medula. Recommended concentration: 4-6μg/mL. Paraffin embedded Human Kidney and Spleen. Recommended concentration: 5μg/mL.

Prediction Reactivity

Rat

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_112475.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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