TIA1 Antibody

Product Specifications

Product Name Alternative

TIA1, TIA1 cytotoxic granule-associated RNA binding protein , TIA1 cytotoxic granule-associated RNA-binding protein, TIA1 protein, cytotoxic granule-associated RNA-binding protein, p40-TIA-1 (containing p15-TIA-1)

UniProt

P31483

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-PKSTYESNTKQ

Target

TIA1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour. An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Thyroid. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5μg/mL.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_071505.1, NP_071320.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.