TRIM24 Antibody

Product Specifications

Product Name Alternative

TRIM24, TIF1, transcriptional intermediary factor 1, PTC6, TF1A, TIF1A, Tif1a, hTIF1, TIF1ALPHA, transcriptional intermediary factor 1 alpha, tripartite motif protein 24, tripartite motif-containing 24, RNF82

UniProt

O15164

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-KRLKSIEERQLLK

Target

TRIM24

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 120kDa band in human kidney and heart lysates at 1μg/mL, however prominent bands were also seen at 25kDa and 45kDa. Please note that currently we cannot find an explanation in the literature for some of the bands we observe given the predicted sizes of approx. 117kDa and 121kDa according to NP_003843 and NP_056989. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Breast cancer shows nuclear staining in the distorted lobules. Recommended concentration, 4-6μg/mL.

Prediction Reactivity

Bovine

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_003843.3, NP_056989.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.