TRPC6 Antibody
TRPC6 Antibody
Product Specifications
Product Name Alternative
TRPC6, transient receptor potential cation channel, subfamily C, member 6, HGNC:12338, TRP6, transient receptor potential channel 6, FLJ11098, FLJ14863, FSGS2, TRP6
UniProt
Q9Y210
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-EKLSMEPNQEETN
Target
TRPC6
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 100kDa band observed in lysates of cell line A549 (calculated MW of 106kDa according to NP_004612.2) . Preliminary experiments also showed 100kDa in Human Lung lysates and in lysates of cell line HEK293 .The observed molecular weights correspond to different antibodies from other commercial sources. Recommended concentration: 0.3-1μg/mL. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine, Porcine
Tested Applications
ELISA, FC, WB
NCBI Accession Number
NP_004612.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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