What is the catalog number for UBE2C Antibody?

The catalog number for UBE2C Antibody is orb1246818.

What sizes are available for UBE2C Antibody?

UBE2C Antibody is available in the following size(s): 0.1 mg.

Is UBE2C Antibody in stock?

UBE2C Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

UBE2C Antibody

Product Specifications

Product Name Alternative

UBE2C, UBCH10, dJ447F3.2, ubiquitin-conjugating enzyme E2C, ubiquitin-protein ligase C, ubiquitin carrier protein E2-C, cyclin-selective ubiquitin carrier protein, mitotic-specific ubiquitin-conjugating enzyme, RP3-447F3.1

UniProt

O00762

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-QETYSKQVTSQEP

Target

UBE2C

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx.19KDa band observed in lysates of cell lines HeLa and HEK293 (calculated MW of 19.7kDa according to NP_008950.1) . Recommended concentration: 1-3μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10μg/mL. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads.

Tested Applications

ELISA, FC, IF, IP, WB

NCBI Accession Number

NP_861516.1, NP_861518.1, NP_001268670.1, NP_861517.1, NP_861515.1, NP_008950.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.