UNC13D Antibody
UNC13D Antibody
Product Specifications
Product Name Alternative
UNC13D, Munc13-4, unc-13 homolog D (C. elegans), FHL3, HLH3, HPLH3, unc-13 homolog D
UniProt
Q70J99
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-ETQKHKKDLHPLFD
Target
UNC13D
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx. 105kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123kDa according to NP_954712.1) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5μg/mL. In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data kindly provided by Micheal Tuvim, University of Texas MD Anderson Cancer Center, Houston, USA. Recommended concentration: 1-2μg/mL.
Prediction Reactivity
Bovine, Canine, Porcine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_954712.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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