VPS11 Antibody

Product Specifications

Product Name Alternative

VPS11, vacuolar protein sorting 11 (yeast), HGNC:14583, END1, PEP5, RNF108, hVPS11 , vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting protein 11, vacuolar protein sorting 11 homolog (S. cerevisiae)

UniProt

Q9H270

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-HALQEKDTQTKLE

Target

VPS11

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 75kDa band in human duodenum, colon and placenta lysates after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108kDa according to NP_068375.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Kidney shows brushborder staning in PCT cells and Human Pancreas shows cytoplasm staining of beta cells in islets of Langerhans. Recommended concentration, 3-6μg/mL.

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_068375.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.