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VTCN1 Antibody

VTCN1 Antibody

Product Specifications

Product Name Alternative

B7-H4, B7H4, B7S1, B7X, B7h.5, FLJ22418, PRO1291, RP11-229A19.4, VCTN1, T cell costimulatory molecule B7x, immune costimulatory protein, V-set domain containing T cell activation inhibitor 1

UniProt

Q7Z7D3

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-SKGKGNANLEYK

Target

VTCN1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 26+21kDa bands observed in HEK293 cell lysates, which were both successfully blocked by incubation with the immunizing peptide. An approx 26kDa and 21kDa band observed in Human Adrenal Gland lysates, and approx 27kDa in Human Pancreas lysates (calculated MW of 30.9kDa according to NP_078902.2 isoform 1 and 20.7kDa according to NP_001240778.1 isoform 2) . Recommended concentration: 0.1-1μg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes/cell junctions of MCF7 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, FC, IF, WB

NCBI Accession Number

NP_078902.2, NP_001240778.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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