WNT3 Antibody
WNT3 Antibody
Product Specifications
Product Name Alternative
WNT3 , wingless-type MMTV integration site family, member 3 , INT4, MGC131950, MGC138321, MGC138323 , WNT-3 proto-oncogene protein precursor
UniProt
P56703
Reactivity
Human
Immunogen
The immunogen for this antibody is: CGRGHNTRTEKRKEK
Target
WNT3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration: 2-4μg/mL.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_110380.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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