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WNT3 Antibody

WNT3 Antibody

Product Specifications

Product Name Alternative

WNT3 , wingless-type MMTV integration site family, member 3 , INT4, MGC131950, MGC138321, MGC138323 , WNT-3 proto-oncogene protein precursor

UniProt

P56703

Reactivity

Human

Immunogen

The immunogen for this antibody is: CGRGHNTRTEKRKEK

Target

WNT3

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat Brain for this particular batch.Immunohistochemistry:In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration: 2-4μg/mL.

Prediction Reactivity

Canine, Mouse, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_110380.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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