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GAGED2 Antibody

GAGED2 Antibody

Product Specifications

Product Name Alternative

GAGED2, XAGE1, XAGE-1, G antigen, family D, 2, XAGE-1 protein

UniProt

Q9HD64

Reactivity

Human

Immunogen

The immunogen for this antibody is: CGFGFRRQGEDNT

Target

GAGED2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the predicted size of approx. 8.0kDa according to NP_597673. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human testis shows polar cytoplasm staining in primary spermatocytes. Recommended concentration, 3-6μg/mL.

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_597673.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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