BHMT Antibody
BHMT Antibody
Product Specifications
Product Name Alternative
BHMT, betaine-homocysteine methyltransferase
UniProt
Q93088
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-EQQLKELFEKQK
Target
BHMT
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 45kDa band observed in Rat Liver lysates (calculated MW of 44.9kDa according to human NP_001704.1, 45.0kDa according to mouse NP_057877.1 and rat NP_110477.1) . This product has been successfully used in WB on Human (PMID: 27320863) . This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966) . This product has been successfully used in WB on Rat (Choi et al. Journal of Functional Foods 44 (2018) 65-73) . In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Kidney. Recommended concentration: 5μg/mL.
Prediction Reactivity
Canine
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001704.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
GTR15215837 1 Vial
Lentiviral mouse Alcam shRNA (UAS) - Lentiviral mouse Alcam shRNA (UAS) (25)
Sign In for Pricing
View Details 