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CLIC4 Antibody

CLIC4 Antibody

Product Specifications

Product Name Alternative

CLIC4, chloride intracellular channel 4, CLIC4L, DKFZP566G223, FLJ38640, H1, huH1, p64H1 , chloride intracellular channel 4 like

UniProt

Q9Y696

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: NGLKEEDKEPLIE-C

Target

CLIC4

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Human Kidney and Placenta lysates (calculated MW of 28.8kDa according to NP_039234.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Edwards et al, Am J Physiol. 1999 Mar;276 (3 Pt 2) :F398-408.; PMID: 10070163) . Recommended concentration: 0.1-0.3μg/mL.Immunohistochemistry:Paraffin embedded Human Heart. Recommended concentration: 5μg/mL.Immunofluorescence: Min6 cells transiently expressing mouse Clic4 shows cytoplasmic staining of two cells. Recommended concentration, 5μg/mL with overnight incubation.

Prediction Reactivity

Canine, Rat

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_039234.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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