DIO2 Antibody

Product Specifications

Product Name Alternative

DIO2, deiodinase, iodothyronine, type II , 5DII, D2, SelY, TXDI2 , thyroxine deiodinase, type II, type 2 iodothyronine deiodinase, type II iodothyronine deiodinaseN: type-II 5'deiodinase

UniProt

Q92813

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: EVKKHQNQEDRC

Target

DIO2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Immunohistochemistry:Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3μg/mL. Data obtained from customer.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Porcine, Rat

Tested Applications

ELISA, FC, IF, IHC

NCBI Accession Number

NP_001007024.1, NP_054644.1, NP_000784.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.