DNMT1 Antibody
DNMT1 Antibody
Product Specifications
Product Name Alternative
DNMT1, DNA (cytosine-5-) -methyltransferase 1, CXXC9, DNMT, FLJ16293, MCMT, MGC104992, DNA methyltransferase 1
UniProt
P26358
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-RFESPPKTQPTEDN
Target
DNMT1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 170kDa band observed in lysates of cell line Jurkat (calculated MW of 183kDa according to NP_001370.1) . Recommended concentration: 0.5-1.5μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse for this particular batch.Immunohistochemistry:In paraffin embedded Human Kidney shows nuclear staining in PCT. Recommended concentration: 3-6μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_001370.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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