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MSH4 Antibody

MSH4 Antibody

Product Specifications

Product Name Alternative

MSH4, mutS homolog 4 (E. coli), RP11-262K24.2, mutS homolog 4

UniProt

O15457

Immunogen

The immunogen for this antibody is: C-ETNSSTFMKEMKE

Target

MSH4

Clonality

Polyclonal

Conjugation

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave bands at approx 60kDa and 35kDa in Human Testis, Ovary and Uterus lysates and in Mouse and Rat Testis lysates after 0.1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 105kDa according to NP_002431.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Human, Mouse

Tested Applications

ELISA

NCBI Accession Number

NP_002431.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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