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Stra6 Antibody

Stra6 Antibody

Product Specifications

Product Name Alternative

STRA6, stimulated by retinoic acid gene 6 homolog (mouse), FLJ12541, MCOPS9, PP14296 , stimulated by retinoic acid gene 6 homolog

UniProt

O70491

Reactivity

Mouse, Rat

Immunogen

The immunogen for this antibody is: C-QAFRKAALTSAKAN

Target

Stra6

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80-90kDa band observed in Rat Testis lysates, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 73.8kDa according to Rat NP_001025095.1) . Recommended concentration: 1-2μg/mL. Primary incubation 1 hour at room temperature. An approx. 90kDa band was also observed in 3T3-L1 adipocytes reduced by stra6 siRNA. The 90kDa is stained after retrovirally overexpressing Mouse Stra6 and not after overexpressing Human Stra6 in 3T3 preadipocytes (calculated MW of 73.8kDa according to Mouse NP_033317.2) . Primary incubation was 3 hours.

Tested Applications

ELISA, WB

NCBI Accession Number

NP_033317.2, NP_001025095.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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