SPHK1 Antibody
SPHK1 Antibody
Product Specifications
Product Name Alternative
SPHK1, sphingosine kinase 1, SPHK, SK1, SKase
UniProt
Q9NYA1
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-DVDLESEKYRRLGE
Target
SPHK1
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2) . Recommended concentration: 2-3μg/mL.Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, IP, WB
NCBI Accession Number
NP_001136073.1, NP_068807.2, NP_892010.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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