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SPHK1 Antibody

SPHK1 Antibody

Product Specifications

Product Name Alternative

SPHK1, sphingosine kinase 1, SPHK, SK1, SKase

UniProt

Q9NYA1

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-DVDLESEKYRRLGE

Target

SPHK1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2) . Recommended concentration: 2-3μg/mL.Immunoprecipitation: EB05266 has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, IP, WB

NCBI Accession Number

NP_001136073.1, NP_068807.2, NP_892010.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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