Clu Antibody
Clu Antibody
Product Specifications
Product Name Alternative
ApoJ, clusterin, Clu, AI893575, Cli, D14Ucla3, SP-40, Sgp-2, Sgp2, Sugp-2, Apolipoprotein J, complement lysis inhibitor, testosterone repressed prostate message
UniProt
Q06890
Reactivity
Human, Rat
Immunogen
The immunogen for this antibody is: C-EKALQEYRRKSRAE
Target
Clu
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 38kDa band observed in Rat Brain and Human Testis lysates. (calculated MW of 51.4kDa according to Rat XP_006252156.1) The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources and the literature (Miyata et al, PMID: 11560857) . The 38kDa band is thought to correspond to the alpha chain of APOJ. Recommended concentration: 0.1-1μg/mL. An additional band of unknown identity was also consistently observed at 28kDa in Rat Brain. This band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Mouse
Tested Applications
ELISA, IF, WB
NCBI Accession Number
NP_038520.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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