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Clu Antibody

Clu Antibody

Product Specifications

Product Name Alternative

ApoJ, clusterin, Clu, AI893575, Cli, D14Ucla3, SP-40, Sgp-2, Sgp2, Sugp-2, Apolipoprotein J, complement lysis inhibitor, testosterone repressed prostate message

UniProt

Q06890

Reactivity

Human, Rat

Immunogen

The immunogen for this antibody is: C-EKALQEYRRKSRAE

Target

Clu

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 38kDa band observed in Rat Brain and Human Testis lysates. (calculated MW of 51.4kDa according to Rat XP_006252156.1) The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources and the literature (Miyata et al, PMID: 11560857) . The 38kDa band is thought to correspond to the alpha chain of APOJ. Recommended concentration: 0.1-1μg/mL. An additional band of unknown identity was also consistently observed at 28kDa in Rat Brain. This band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Mouse

Tested Applications

ELISA, IF, WB

NCBI Accession Number

NP_038520.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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